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tet operator plasmid  (Addgene inc)


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    Structured Review

    Addgene inc tet operator plasmid
    Tet Operator Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tet+operator+plasmid/pLVX-EF1a-tetOn-IRES-G418+(EtO)+(Plasmid+%2384776)/bio_rxiv__2025__04__14__648806-231-46-48
    Average 93 stars, based on 8 article reviews
    tet operator plasmid - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Lentivirus packaging system comprising a synthetic positive feedback loop
    Article Snippet: .. pCSII-SYN-Venus, pCSII-Sox2, pCMV-VSV-G-RSV-rev plasmids were obtained A Miyoshi (Keio University). pCSII mVenus-hGeminin and pCSII-mCherry-hCDT plasmids were obtained from A. Miyawaki (RIKEN BSI). pcDNA3-mRFP plasmid was obtained from D. Golenbock (University of Massachusetts Medical School, Addgene plasmid #13032). tet operator plasmid was obtained from L. Chin (University of Texas, Addgene plasmid #8901). .. FUW-OSKM and TetO-FUW-OSKM were obtained from R. Jaenisch (Whitehead Institute, Addgene plasmid #20328, #20321). pSMPUW, pCgpV, and pRSV-rev were obtained from CellBioLabs.

    Article Title: Lentivirus packaging system comprising a synthetic positive feedback loop
    Article Snippet: .. Materials pCSII-SYN-Venus, pCSII-Sox2, pCMV-VSV-G-RSV-rev plasmids were obtained from A Miyoshi (Keio University). pCSII-mVenus-hGeminin and pCSII-mCherry-hCDT plasmids were obtained from A. Miyawaki (RIKEN BSI). pcDNA3-mRFP plasmid was obtained from D. Golenbock (University of Massachusetts Medical School, Addgene plasmid #13032). tet operator plasmid was obtained from L. Chin (University of Texas, Addgene plasmid #8901). .. FUW-OSKM and TetO-FUW-OSKM were obtained from R. Jaenisch (Whitehead Institute, Addgene plasmid #20328, #20321). pSMPUW, pCgpV, and pRSV-rev were obtained from CellBioLabs.

    Article Title: Designed NGF mimetics with reduced nociceptive signatures in neurons
    Article Snippet: Cells were passaged every 3-4 days using trypsin-EDTA (Gibco, Cat #25300054), maintained at 37°C in a humidified incubator and routinely tested for mycoplasma. .. For conversion to neurons, stable lines were generated by transducing two lentiviruses produced in HEK293FT cells with packaging plasmids psPAX2 and pMD2.G following the Addgene protocol: The first one containing pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A), a gift from Fred Gage (Addgene plasmid # 84777), a the second one a tet-operator plasmid (Addgene # 84776), followed by 4 days of puromycin (1μg/ml) and G418 (150 μg/ml) selection. ..

    Generated:

    Article Title: Designed NGF mimetics with reduced nociceptive signatures in neurons
    Article Snippet: Cells were passaged every 3-4 days using trypsin-EDTA (Gibco, Cat #25300054), maintained at 37°C in a humidified incubator and routinely tested for mycoplasma. .. For conversion to neurons, stable lines were generated by transducing two lentiviruses produced in HEK293FT cells with packaging plasmids psPAX2 and pMD2.G following the Addgene protocol: The first one containing pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A), a gift from Fred Gage (Addgene plasmid # 84777), a the second one a tet-operator plasmid (Addgene # 84776), followed by 4 days of puromycin (1μg/ml) and G418 (150 μg/ml) selection. ..

    Produced:

    Article Title: Designed NGF mimetics with reduced nociceptive signatures in neurons
    Article Snippet: Cells were passaged every 3-4 days using trypsin-EDTA (Gibco, Cat #25300054), maintained at 37°C in a humidified incubator and routinely tested for mycoplasma. .. For conversion to neurons, stable lines were generated by transducing two lentiviruses produced in HEK293FT cells with packaging plasmids psPAX2 and pMD2.G following the Addgene protocol: The first one containing pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A), a gift from Fred Gage (Addgene plasmid # 84777), a the second one a tet-operator plasmid (Addgene # 84776), followed by 4 days of puromycin (1μg/ml) and G418 (150 μg/ml) selection. ..

    Selection:

    Article Title: Designed NGF mimetics with reduced nociceptive signatures in neurons
    Article Snippet: Cells were passaged every 3-4 days using trypsin-EDTA (Gibco, Cat #25300054), maintained at 37°C in a humidified incubator and routinely tested for mycoplasma. .. For conversion to neurons, stable lines were generated by transducing two lentiviruses produced in HEK293FT cells with packaging plasmids psPAX2 and pMD2.G following the Addgene protocol: The first one containing pLVX-TREtight-Ngn2:2A:Ascl1-PGK-Puro (XTP-N2A), a gift from Fred Gage (Addgene plasmid # 84777), a the second one a tet-operator plasmid (Addgene # 84776), followed by 4 days of puromycin (1μg/ml) and G418 (150 μg/ml) selection. ..



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